colony hybridisationmedical dictionary

<molecular biology> A genetics lab technique used to identify which colonies of bacteria on an agar plate contain a particular sequence of DNA or a particular gene.

The technique involves pressing a nylon or nitrocellulose membrane onto the plate so that each colony contributes a small smudge of itself to the membrane, then treating the membrane with chemicals and heat, then washing the membrane with a labelled probe to find the specific DNA sequence. The smudges which are indicated by the probe are then compared back to the colonies on the agar plate. This technique is often used in conjunction with experiments involving the making of genomic libraries.

(09 Oct 1997)