site-specific DNA-methyltransferase (adenine-specific)medical dictionary

<enzyme>

<cell biology> An enzyme responsible for producing a species-characteristic methylation pattern on adenine residues in a specific short base sequence in the host cell DNA. The enzyme catalyses the methylation of DNA adenine in the presence of s-adenosyl-l-methionine to form DNA containing 6-methylaminopurine and s-adenosyl-l-homocysteine.

Registry number: EC 2.1.1.72

(12 Dec 1998)

site-specific DNA methyltransferase (cytosine-specific)medical dictionary

<enzyme>

<cell biology> An enzyme responsible for producing a species-characteristic methylation pattern on cytosine residues in a specific short base sequence in the host cell's DNA. The enzyme catalyses the methylation of DNA cytosine in the presence of s-adenosyl-l-methionine to form s-adenosyl-l-homocysteine and DNA containing 5-methylcytosine.

Registry number: EC 2.1.1.73

(12 Dec 1998)

site-specific mutagenesismedical dictionary

<cell culture, molecular biology> An in vitro technique in which an alteration is made at a specific site in a DNA molecule, which is then reintroduced into a cell. Various techniques are used, for the cell biologist, a very powerful approach to determining which parts of a protein or nucleotide sequence are critical to function.

This entry appears with permission from the Dictionary of Cell and Molecular Biology

(11 Mar 2008)

site specific mutationmedical dictionary

An alteration of the structure of a gene at a specific sequence, usually referring to experimentally produced changes in gene sequence.

(05 Mar 2000)

site-specific recombinationmedical dictionary

<molecular biology> A type of recombination that occurs between two specific short DNA sequences present in the same or in different molecules. An example is the integration and excision of _ prophage.

This entry appears with permission from the Dictionary of Cell and Molecular Biology

(11 Mar 2008)